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Sunday, July 5, 2020 | History

3 edition of A transgenic TCR model for studying the veto phenomenon. found in the catalog.

A transgenic TCR model for studying the veto phenomenon.

Nazik Hammad

A transgenic TCR model for studying the veto phenomenon.

by Nazik Hammad

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  • 24 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1995.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination1 microfiche : negative. --
ID Numbers
Open LibraryOL17247854M
ISBN 10061202069X
OCLC/WorldCa46512463

Transgenic Mouse Models—A Seminal Breakthrough in Oncogene Research Click here for more information or to buy the book. osteosarcoma model; RIP-Tag model used to study the role of angiogenesis in tumor progression MMTV-PyV middle T-antigen breast cancer model.   Firstly, adoptive transfer of splenocytes from healthy KRN TCR transgenic mice (B, or B x N) into I-Ag7 positive or negative, RAG1 or TCR apha deficient recipients, revealed that T cells, B cells and I-Ag7 expression were essential for arthritis : Andrew Cope.

  Engineering T Cells in Cancer Therapy: CAR-T, TCR-T and Others | Presented by Dr. Naoto Hirano - Duration: Princess Margaret Cancer Centre 3, views Customized Transgenic / Genetically Modified Research Models. Customized genetically modified (transgenic) models heavily impact the scientific value of your studies, as the models are designed to specifically address your scientific questions while avoiding the unrevealed caveats possible in genetically modified at genOway: Genetically modified mice, rats and cell lines.

Advances in transgenic animal models and techniques Se´verine Me´noret. Laurent Tesson. Se´verine Remy. Claire Usal. Laure-He´le`ne Ouisse. Lucas Brusselle. Vanessa Chenouard. Ignacio Anegon Received: 26 July /Accepted: 31 July Springer International Publishing AG Abstract On May 11th and 12th was held in. Tolerance induction by veto CTLs in the TCR transgenic 2C mouse model. II Deletion of effector cells by Fas-Fas ligand apoptosis. J Immunol., , Reich-Zeliger, S., Bachar-Lustig, E., Gan, J., Reisner, Y. () Tolerance induction by veto CTLs in the TCR transgenic 2C mouse model.I Relative reactivity of different veto cell. J.


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A transgenic TCR model for studying the veto phenomenon by Nazik Hammad Download PDF EPUB FB2

Abstract. Cellular immune responses are crucial both for protective immunity against salmonellosis, and for the immunogenicity of oral vaccines based on avirulent live Salmonella as antigen carriers. The crucial early steps of T cell induction are difficult to investigate in conventional animals, but recently developed T cell receptor (TCR)-transgenic models allow visualization of antigen Cited by:   This is in contrast to the FH transgenic mouse model that bears MHC class I restricted murine tyrosinase TCR or MHC class II-restricted TCR transgenic mouse model in which CD4 + T cells recognize a novel epitope in TRP It is notable that despite the difference in anchor residues between murine and human tyrosinase epitopes, the TILI transgenic TCR bearing T cells were able to recognize murine epitope but vitiligo Cited by: Therefore, it is unclear whether TCR-transgenic cells may be used for cDNA library screening with the same efficacy as original T cell clones.

In this study we demonstrate for the first time the applicability of TCR-transgenic primary T cells for antigen discovery via cDNA library by: 7.

t cell receptor transgenic mouse as a model system to study t lymphocyte development Antigen recognition by the T cell is accomplished by the TCR. TCR molecules are composed of two chains, designated α and β, each of which has variable (V) and constant (C) regions (5).Author: Dennis Y.

Loh. The direct assay of veto CTLs in the 2C mouse model enables monitoring, by FACS, the fate of the TCR transgenic effector CD8(+) T cells, the transgene of which can be stained with clonotypic Ab 1B2. The data do indicate that tissue morphology is normal in the mB29b-TCR transgenic mouse.

Furthermore, no spontaneous autoimmune disease was observed in these young TCR transgenic mice. With this new TCR transgenic mouse, we are now able to study the properties of naive T cells differentiating and proliferating into effector and by: 5.

Genetic modification of T lymphocytes with T-cell receptor (TCR) genes provides a novel tool for adoptive immunotherapy. However, the efficiency of full-length TCR (flTCR)-transduced T cells could be limited by factors such as incorrect pairing between exogenous and endogenous TCR chains and downregulation of the CD3 complex.

current challenges of TCR gene therapy is the optimiz-ation of TCRa and b transgene pairing to enhance the functional avidity of therapeutic T cells. Recently, var-ious genetically modified TCRs have been developed that enhance TCR pairing and minimize mispairing, i.e. pairing between transgenic and endogenous TCR Size: KB.

The Transgenic LCMV-P14 T Cell Receptor transgenic mouse was made by Dr. Hanspeter Pircher and colleagues in at the Department of Experimental Pathology in Zurich, Switzerland.

The transgenic line was created by coinjection of the P14 Tcrα and Tcrβ gene constructs into. An experimental system was established to study in vivo T-cell receptor αβ (TCR) mutations in murine CD4 + T-lymphocytes. The frequency of TCR-defective mutant T-cells that have the CD3 − 4 + surface phenotype, was measured using two-color flow cytometry of splenic T-cells passed through nylon wool.

The spontaneous TCR mutant frequency (MF) in BALB/c mice (×10 −4) was significantly Cited by: To study the response of T regs to antigen in vivo, we have taken advantage of a murine model that allows the production of a large number of CD25 + T cells with regulatory function that bear a transgenic TCR.

Using the clonotypic antibody to identify these cells after adoptive transfer to nontransgenic recipients, we reveal a key difference between the responsiveness of T regs to encounter Cited by: Among the current challenges of TCR gene therapy is the optimization of TCRα and β transgene pairing to enhance the functional avidity of therapeutic T cells.

Recently, various genetically modified TCRs have been developed that enhance TCR pairing and minimize mispairing, i.e. pairing between transgenic and endogenous TCR by: Genetic Tools to Study T Cell Development. with the generation and use of TCR transgenic mice for studying all aspects of T-cell biology.

a transgenic mouse model system whereby memory T. Summary: Adoptive transfer of TCR‐transgenic T cells uniformly expressing an identifiable TCR of known peptide/MHC specificity can be used to monitor the in vivo behavior of antigen‐specific T cells.

We have used this system to show that naive T cells are initially activated within the T‐cell zones of secondary lymphoid tissue to prohferate in a B7‐dependent by: Antigen-specific regulatory T cells (Tregs) directed at self-antigens are difficult to study since suitable specific tools to isolate and characterize these cells are lacking.

A T cell receptor (TCR)-transgenic mouse would generate possibilities to study such ­antigen-specific T cells. As was shown previously, immunization with the mycobacterial heat shock protein (Hsp) derived peptide B29 Cited by: 5.

Summary. Transgenic animals have become a key tool in functional genomics to generate models for human diseases and validate new drugs.

Transgenesis includes the addition of foreign genetic information to animals and specific inhibition of endogenous gene by: The Rec-HY TCR transgenic model allowed us to clearly identify and study self-reactive thymocytes that have rearranged their TCR but have not yet been deleted.

Consistent with having received stronger TCR signals, self-reactive thymocytes had increased levels of CD69 and CD5 and lower levels of TCR Cited by:   The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to by: 1.

Mice. To generate pMDMspecific TCR transgenic mice the Vα5 and Vβ7 TCR chains from a high-avidity allo-major histocompatibility complex (MHC)-restricted pMDMspecific CTL clone 26 – 28 were cloned into a CD2-vector (courtesy of Dr D. Kioussis, National Institute for Medical Research, London, UK) in preparation for microinjection into (C57BL/6 × CBA) F 1 (H2 bxk) oocytes to produce Cited by: 2.

Genetic modification of T lymphocytes with T-cell receptor (TCR) genes provides a novel tool for adoptive immunotherapy. However, the efficiency of full-length TCR (flTCR)-transduced T Cited by:.

Since the expression of a transgenic rearranged TCR β gene has long been known to efficiently suppress the rearrangement of endogenous TCR β genes, crossing TCR Tg mice with TCR β knockout mice could have been a futile by:   T-cell receptor (TCR)-engineered T cells are a novel option for adoptive cell therapy used for the treatment of several advanced forms of cancer.

Work using TCR-engineered T cells began more than two decades ago, with numerous preclinical studies showing that such cells could mediate tumor lysis and eradication. The success of these trials provided the foundation for clinical trials, including Cited by: This transgenic model system also provides a tool for studying the effects of tolerogenic protocols on alloreactive CD4 + T cells in vivo.

To this end we validated our model by investigating the role of the CD28‐B7 costimulatory pathway in MHC class II alloreactive CD4 + T‐cell responses using CTLA4Ig, which inhibits the interaction of CD28 and CTLA‐4 with B7‐1 and B7‐ by: